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Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine
Published on: May 21, 2012
Reduced CD1d expression in colonic epithelium in microscopic colitis.
Yimin Ge1, Bill A Rampy, Hanlin L Wang
1Department of Pathology, University of Texas Medical Branch, Galveston 77555-0588, USA.
Applied Immunohistochemistry & Molecular Morphology : AIMM
|August 26, 2006
Summary
Microscopic colitis is linked to reduced CD1d expression in the gut, a key molecule for mucosal immunity. This decrease correlates with more T lymphocytes in the colon lining.
Area of Science:
- Immunology
- Gastroenterology
- Cell Biology
Background:
- CD1d is a major histocompatibility complex class I-like molecule.
- It plays a role in gastrointestinal mucosal immunity by presenting antigens to T lymphocytes.
- Reduced CD1d expression may impact immune responses in the gut.
Purpose of the Study:
- To investigate CD1d expression levels in microscopic colitis.
- To compare CD1d expression in lymphocytic colitis and collagenous colitis with normal colonic mucosa.
Main Methods:
- Immunohistochemical analysis of colon biopsies from patients with lymphocytic colitis, collagenous colitis, and healthy controls.
- Monoclonal antibodies against CD1d, CD3, CD4, and CD8 were used.
- CD1d staining was graded, and intraepithelial lymphocytes were quantified.
Main Results:
- CD1d was expressed in normal colonic epithelial cells, mainly on basolateral membranes.
- CD1d expression was significantly reduced in both lymphocytic colitis and collagenous colitis.
- A significant increase in intraepithelial CD3/CD8 lymphocytes was observed in microscopic colitis.
Conclusions:
- Microscopic colitis is associated with decreased epithelial CD1d expression.
- CD1d is an important immunoregulatory molecule in the gastrointestinal tract.
- The findings suggest a role for CD1d in the pathogenesis of microscopic colitis.
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