Demonstration of enzymes related to myelinogenesis in established human brain cell cultures

CNP, CGalT and PAPS-CST, enzymes involved in myelinogenesis were demonstrated in cultures derived from human brain. Little or no CNP activity could be found in cultures derived from non-neurogenic tissue. Transformation of brain cultures by PML-SV40 virus resulted in a 3 to 10-fold increase in the specific activity of CNP.