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Related Experiment Videos

Alveolar epithelial cell keratin expression during lung development.

J Woodcock-Mitchell1, J J Mitchell, S E Reynolds

  • 1Department of Physiology and Biophysics, College of Medicine, University of Vermont, Burlington 05405.

American Journal of Respiratory Cell and Molecular Biology
|June 1, 1990
PubMed
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Keratin 18 expression in rat lung epithelial cells increases significantly during development, particularly in type II cells during active alveolarization. Keratin 19 is primarily found in adult type II pneumocytes.

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Pulmonary Medicine

Background:

  • Keratin expression and organization are crucial for understanding epithelial cell differentiation in developing and remodeling tissues.
  • Keratin 18 and Keratin 19 are of particular interest due to their potential roles in alveolar epithelial cell transitions.

Purpose of the Study:

  • To examine the distribution of keratin 18 and keratin 19 in rat lung epithelial cells from gestation to adulthood.
  • To investigate the role of these keratins in lung development and remodeling.

Main Methods:

  • Monoclonal antibodies (24A3 for keratin 18, AE3 for basic keratins) were used to examine keratin distribution.
  • Immunohistochemistry and immunoblot analysis were employed.

Main Results:

Related Experiment Videos

  • Keratin 18 expression begins around day 16 of gestation, peaks in the parenchyma between postnatal days 5-10, and is mainly at the periphery of adult type II cells.
  • Keratin 19 is detected later in gestation (day 19) and predominantly stains adult type II pneumocytes throughout the cell.
  • AE3 antibody showed similar staining patterns to keratin 19.

Conclusions:

  • Keratin 18 is highly expressed in type II cells during periods of intense proliferation and alveolarization in developing rat lungs.
  • These findings correlate with previous observations on keratin expression in response to lung injury.