Related Experiment Video
Updated: Jul 20, 2026
Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets
Published on: November 22, 2024
Flow cytometric evaluation of platelet activation in chronic autoimmune thrombocytopenia
Simon Panzer1, Lisa Höcker, Rainer Vormittag
1Clinic for Blood Group Serology, Department of Internal Medicine, Medical University of Vienna, Vienna, Austria. simon.panzer@meduniwien.ac.at
Platelets from 42 patients (platelet counts median 42x10(9)/L, range 3-223x10(9)/L) with chronic idiopathic autoimmune thrombocytopenia (cAITP) were investigated for P-Selectin expression and PAC-1 binding. The results showed that the levels of P-Selectin positive platelets (n=20) were higher in cAITP than in controls (P<0.0001), and correlated with platelet counts (P=0.04). PAC-1 binding was increased in only six patients, and not correlated with platelet counts. There was no correlation of P-Selectin or PAC-1 with detectable platelet antibodies. Thus, platelets are activated in cAITP, but platelets, characterized by PAC-1 binding, are rare. These are either needed to maintain vascular integrity, or underwent premature sequestration.
Platelets from 42 patients (platelet counts median 42x10(9)/L, range 3-223x10(9)/L) with chronic idiopathic autoimmune thrombocytopenia (cAITP) were investigated for P-Selectin expression and PAC-1 binding. The results showed that the levels of P-Selectin positive platelets (n=20) were higher in cAITP than in controls (P<0.0001), and correlated with platelet counts (P=0.04). PAC-1 binding was increased in only six patients, and not correlated with platelet counts. There was no correlation of P-Selectin or PAC-1 with detectable platelet antibodies. Thus, platelets are activated in cAITP, but platelets, characterized by PAC-1 binding, are rare. These are either needed to maintain vascular integrity, or underwent premature sequestration.
