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Updated: Jul 20, 2026
08:12
Depolymerizable Olefinic Polymers Based on Fused-Ring Cyclooctene Monomers
Published on: December 16, 2022
Poly(cyclooctene)-based monolithic columns for capillary high performance liquid chromatography prepared via
Bettina Schlemmer1, Christina Gatschelhofer, Thomas R Pieber
1Leibniz Institut für Oberflächenmodifizierung e.V. (IOM), Permoserstrasse 15, D-04318 Leipzig, Germany.
Journal of Chromatography. A
|August 29, 2006
Summary
Novel monolithic capillary HPLC columns were synthesized using ring-opening metathesis polymerization (ROMP). These columns demonstrate excellent reproducibility and long-term stability for protein analysis, showing minimal performance alteration over 1000 runs.
Area of Science:
- Analytical Chemistry
- Polymer Chemistry
- Chromatography
Background:
- High-performance liquid chromatography (HPLC) is a cornerstone of analytical chemistry.
- Development of robust and reproducible monolithic stationary phases is crucial for efficient separations.
- Capillary HPLC offers advantages in sensitivity and reduced solvent consumption.
Purpose of the Study:
- To synthesize and evaluate novel monolithic capillary HPLC columns.
- To assess the reproducibility and stability of these columns for protein analysis.
- To investigate the influence of synthesis parameters on column performance.
Main Methods:
- Monolithic columns were prepared using ring-opening metathesis polymerization (ROMP) of cis-cyclooctene (COE) and tris(cyclooct-4-enyl-1-oxy)methylsilane (CL) monomers.
- Initiator: RuCl(2)(Py)(2)(IMesH(2))(CHC(6)H(5)); Porogens: 2-propanol and toluene.
- Column evaluation involved separating a protein standard (5,800–66,000 g/mol) and assessing retention time reproducibility (RSD 2.9–3.9%).
Main Results:
- Synthesis reproducibility was confirmed with low relative standard deviations in retention times.
- Variations in pyridine concentration significantly impacted monolith morphology, backpressure, and retention times.
- The columns exhibited excellent long-term stability over 1000 runs at 50°C with minimal changes in separation performance.
Conclusions:
- ROMP is a viable method for fabricating robust monolithic capillary HPLC columns.
- The synthesized columns offer reliable and stable performance for protein analysis.
- Further optimization of polymerization kinetics can fine-tune column properties for specific applications.
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