Triple staining of brain tissue for neuronal classification

The described triple staining (PAP, aldehydefuchsin, and cresyl violet) enables the distinction of different neuronal types with regard to their content of neurotransmitters or neuropeptides and their lipofuscin pigmentation respectively. The use of filters improves the contrast of immunohistochemical and lipofuscin staining, and facilitates the differentiation of neurons under the microscope. An additional important point is the advantage that perikaria can be measured in the usual morphometric manner.