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Related Concept Videos

DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

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Related Experiment Video

Updated: Jun 25, 2026

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair
08:15

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

Published on: October 6, 2014

DNA technology.

R S Wong1, E Passaro

  • 1Department of Surgery, UCLA School of Medicine.

American Journal of Surgery
|June 11, 1990
PubMed
Summary
This summary is machine-generated.

Genetic technologies like DNA recombinant technology and gene cloning allow molecular-level disease study and protein production. Future gene therapy promises to repair genetic defects, impacting clinical practice.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Medical Genetics

Background:

  • Advancements in DNA recombinant technology and sequencing enable molecular-level disease understanding.
  • Gene cloning facilitates the production of unlimited quantities of pure protein products.

Purpose of the Study:

  • To explain recent advances in genetic technology.
  • To discuss the clinical relevance and applications of these genetic technologies.

Main Methods:

  • Review of DNA recombinant technology.
  • Analysis of DNA and RNA sequencing techniques.
  • Exploration of gene cloning applications.

Main Results:

  • Diseases can be studied and treated at a molecular level.
  • Pure protein products can be produced in unlimited quantities.
  • Gene therapy is poised to repair genetic defects.

Conclusions:

  • Genetic technologies offer new avenues for disease diagnosis and treatment.
  • Gene therapy holds significant promise for correcting inherited disorders.
  • These advancements are expected to revolutionize clinical practice.