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A baboon model for pregnancy-associated antigens (PAPP-A, PP5, PP14)
M J Sinosich1, V Z Pope, C E Pope
1Department of Obstetrics and Gynaecology, Royal North Shore Hospital, St. Leonards, Australia.
Archives of Gynecology and Obstetrics
|January 1, 1990
Summary
This study investigated pregnancy-associated plasma protein-A (PAPP-A), PP5, and PP14 in baboons, finding PAPP-A shows significant cross-reactivity, supporting its use in baboon models for feto-maternal signal research.
Area of Science:
- Reproductive biology
- Immunology
- Primate models
Background:
- Pregnancy-associated plasma protein-A (PAPP-A), PP5, and PP14 are key feto-maternal signals in humans.
- Understanding these signals in non-human primates can advance reproductive research.
Purpose of the Study:
- To determine the presence and characteristics of PAPP-A, PP5, and PP14 in baboons.
- To evaluate the baboon as a model for studying feto-maternal signals.
Main Methods:
- Radioimmunoassays using human-derived antigens were employed.
- Physicochemical characterization utilized affinity matrices (heparin, zinc chelate, thrombin).
- Immunoreactivity was assessed in placental extracts, blood, follicular fluid, and embryo cultures.
Main Results:
- PAPP-A, PP5, and PP14 immunoreactivity were detected in pregnant baboons.
- PAPP-A exhibited the highest interspecies immunological cross-reactivity between humans and baboons.
- PAPP-A and PP5 were detected in pregnant baboons significantly earlier than PP14.
Conclusions:
- Baboon PAPP-A and PP5 share significant immunological similarities with human counterparts.
- The baboon serves as a viable model for studying feto-maternal signals like PAPP-A and PP5.
- Further research using baboon models can elucidate the roles of these antigens in pregnancy.