Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mechanosensitive channel gating transitions resolved by functional changes upon pore modification.

Jessica L Bartlett1, Yuezhou Li, Paul Blount

  • 1Department of Physiology, University of Texas-Southwestern Medical Center, Dallas, Texas, USA.

Biophysical Journal
|August 29, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrating perceptual cues with mixture-of-experts for low-light image restoration.

Neural networks : the official journal of the International Neural Network Society·2026
Same author

Immune evasion of Omicron variants JN.1, KP.2, and KP.3 to the polyclonal and monoclonal antibodies from COVID-19 convalescents and vaccine recipients.

Antiviral research·2025
Same author

Mechanosensitive channel MscL gating transitions coupling with constriction point shift.

Protein science : a publication of the Protein Society·2024
Same author

Editorial: <i>In silico</i> gating mechanism studies and modulator discovery for MscL.

Frontiers in chemistry·2024
Same author

The Effects of Airflow on the Mechanosensitive Channels of <i>Escherichia coli</i> MG1655 and the Impact of Survival Mechanisms Triggered.

Microorganisms·2023
Same author

Clinical analysis and functional characterization of KCNQ2-related developmental and epileptic encephalopathy.

Frontiers in molecular neuroscience·2023
Same journal

Tau protein differentially affects Piezo1 and Kir2.1 channels in brain capillary endothelial cells.

Biophysical journal·2026
Same journal

Emergent Intercellular Junction Stability during Cyclic Tissue Loading.

Biophysical journal·2026
Same journal

Enhanced-Sampling Simulations Reveal Distinct Intermediates in SARS-CoV-2 FSE Pseudoknot Interconversion.

Biophysical journal·2026
Same journal

Structure-based simulations of the full Flock House virus capsid reveal pathways and energetics of an infection-critical peptide externalization event.

Biophysical journal·2026
Same journal

Quantifying the Peripheral Surface Information Entropy from Conformational Ensembles of Globular Protein-Peptide Complexes.

Biophysical journal·2026
Same journal

Anisotropic unbinding and location-dependent hovering of a kinesin motor head over microtubule.

Biophysical journal·2026
See all related articles

The mechanosensitive channel of large conductance (MscL) protects bacteria from osmotic stress. This study uses single-channel recordings to refine models of MscL gating, revealing pore residue microenvironments.

Area of Science:

  • Biophysics
  • Molecular Biology
  • Microbiology

Background:

  • The mechanosensitive channel of large conductance (MscL) is a critical bacterial osmodaptor protein.
  • Existing models of MscL gating lack resolution and contain inconsistencies.
  • Previous studies identified pore-accessible residues using in vivo SCAM but relied on cell viability for functional assessment.

Purpose of the Study:

  • To investigate the structural transitions of MscL during gating using single-channel recordings.
  • To elucidate the microenvironment of pore residues in different MscL states.
  • To refine existing models of MscL gating and pore domain structure.

Main Methods:

  • Utilized cysteine substitutions in MscL channels.
  • Applied sulfhydryl reagent MTSET in the presence or absence of hypoosmotic shock.

Related Experiment Videos

  • Measured functional modifications via single-channel recordings to assess residue accessibility and channel kinetics.

    • Main Results:

    • Quantified MTSET-induced functional changes in MscL activity.
    • Determined residue availability in closed and open MscL states with higher resolution.
    • Observed alterations in channel kinetics and sensitivity linked to specific residue microenvironments.

    Conclusions:

    • Provided a higher-resolution view of the MscL pore domain and its structural transitions.
    • Validated and refined aspects of existing MscL gating models.
    • Elucidated the microenvironment of key pore residues, advancing understanding of MscL function.