Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Pressure effects on iron-based superconductor CaFe<sub>0.88</sub>Co<sub>0.12</sub>AsF.

Journal of physics. Condensed matter : an Institute of Physics journal·2019

Same author

Pressure tuning of iron-based superconductor Ca<sub>10</sub>(Pt<sub>3</sub>As<sub>8</sub>) ((Fe<sub>0.95</sub>Pt<sub>0.05</sub>)<sub>2</sub>As<sub>2</sub>)<sub>5</sub>.

Journal of physics. Condensed matter : an Institute of Physics journal·2019

Same author

[Impacts of airborne particulate matter and its components on respiratory system health].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2017

Same author

Superconducting fluctuation effect in CaFe0.88Co0.12AsF.

Journal of physics. Condensed matter : an Institute of Physics journal·2016

Same author

Angular dependent torque measurements on CaFe0.88Co0.12AsF.

Journal of physics. Condensed matter : an Institute of Physics journal·2016

Same author

Brucellosis control in northeast China: a long way to go.

Public health·2015

Search research articles

Related Experiment Video

Updated: Jul 20, 2026

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

Nonlinear imaging of embedded microstructures inside transparent materials with laser-induced ionization microscopy.

Y Zhao¹, G Mu, X Zhu

¹Institute of Modern Optics, Nankai University, Key Laboratory of Opto-electronic Information Science and Technology, Education Ministry of China, Tianjin, China.

|August 29, 2006

Summary

We developed a new nonlinear imaging technique using laser-induced plasma emission. This method enhances contrast for microscopic structures in transparent materials, improving upon linear microscopes.

More Related Videos

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Published on: September 3, 2010

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

Published on: January 3, 2017

Related Experiment Videos

Last Updated: Jul 20, 2026

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Published on: September 3, 2010

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

Published on: January 3, 2017

Area of Science:

Materials Science
Optics
Microscopy

Background:

Linear microscopy faces limitations in resolving microscopic structures within transparent materials due to low contrast.
Variations in refractive index often hinder clear visualization of embedded features.

Purpose of the Study:

To introduce a novel nonlinear imaging method for probing microscopic structures in transparent materials.
To demonstrate the capability of resolving elemental or structural variations with high contrast.

Main Methods:

Utilizing femtosecond laser-induced plasma emission as the nonlinear diagnostic tool.
Employing ultrashort laser pulses for fabricating microstructures within optical glass.

Main Results:

Achieved significantly higher contrast ratios compared to ordinary linear microscopes.
Successfully resolved microstructures with differing refractive indices.
Presented examples of microstructures fabricated inside optical glass.

Conclusions:

The developed nonlinear imaging method offers superior contrast for visualizing embedded microstructures.
This technique provides a powerful tool for analyzing transparent materials and laser-fabricated structures.