Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hypercholesterolemia and LDL receptor mRNA expression: modulation by selenium supplementation.

Sanjiv Dhingra1, Mohinder P Bansal

  • 1Department of Biophysics, Punjab University, Chandigarh, 160014, India.

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
|August 29, 2006
PubMed
Summary

Selenium (Se) supplementation can protect against high cholesterol. This study shows that Se boosts LDL receptor (LDL-R) activity and gene expression, improving lipid metabolism in rats.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Generation of a patient-derived iPSC line from a clinically diagnosed MELAS case carrying the mtDNA m.3243A > G variant.

Stem cell research·2026
Same author

Association of stromal cell-derived factor 1α and interferon-γ-induced protein 10 with Fontan pathophysiology.

Canadian journal of physiology and pharmacology·2026
Same author

Association of Fontan Circulation With Gut Microbiome Derived Straight and Branched Short Chain Fatty Acids.

Journal of gastroenterology and hepatology·2026
Same author

iPSC generation from PBMCs of a MELAS patient for mitochondrial dysfunction studies.

Stem cell research·2026
Same author

TCA cycle intermediates as an adjunct strategy for human iPSC-derived cardiomyocyte maturation.

BBA advances·2026
Same author

Autophagy-Cholesterol Axis Remodeling Supports Malignant Progression and Chemoresistance in Glioma.

bioRxiv : the preprint server for biology·2026

Area of Science:

  • Biochemistry
  • Nutritional Science
  • Cardiovascular Research

Background:

  • Selenium (Se) status is linked to cardiovascular health.
  • Hypercholesterolemia, characterized by high cholesterol levels, is a major risk factor for cardiovascular disorders.
  • Low-density lipoprotein receptors (LDL-R) play a crucial role in cholesterol homeostasis.

Purpose of the Study:

  • To investigate the protective effects of selenium supplementation on LDL receptor activity and mRNA expression.
  • To evaluate the impact of Se on lipid metabolism during experimentally induced hypercholesterolemia.

Main Methods:

  • Male Sprague-Dawley rats were fed diets supplemented with 0.2 or 1 ppm Se and/or 2% cholesterol for 3 months.
  • LDL receptor activity was assessed in vivo by measuring the clearance rate of radiolabeled LDL.

Related Experiment Videos

  • LDL receptor mRNA expression was quantified using reverse transcription polymerase chain reaction (RT-PCR).

    • Main Results:

    • Dietary cholesterol significantly decreased both LDL receptor activity and mRNA expression.
    • Supplementation with 1 ppm selenium notably increased LDL receptor activity and mRNA expression.
    • Selenium supplementation demonstrated a dose-dependent effect on LDL receptor regulation.

    Conclusions:

    • Selenium supplementation, particularly at 1 ppm, upregulates LDL receptor activity and mRNA expression in hypercholesterolemic rats.
    • These findings suggest that selenium has therapeutic potential in managing lipid metabolism and mitigating risks associated with hypercholesterolemia.
    • Se supplementation may offer a novel strategy for improving cardiovascular health through enhanced cholesterol clearance.