Related Experiment Video
Updated: Jul 20, 2026

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death
Published on: December 27, 2016
Progesterone-modulated phosphatidylserine externalization in apoptosis and activation of Jurkat cells
Milena Mourdjeva1, Zlatka Popova, Dobroslav Kyurkchiev
1Department of Molecular Immunology, Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, 73 Tzarigradsko shosse, 1113 Sofia, Bulgaria. milena_mourdjeva@hotmail.com
Problem:
During pregnancy the elevated levels of progesterone (Pg) have immunomodulating effects. It is important to follow-up Pg effects on basic biological processes at cell level as apoptosis and activation which was the aim of this study.
Methods Of Study:
Jurkat cells cultured in the presence or absence of Pg were used as a model system. Apoptosis was induced by H(2)O(2) and activation by phorbol myriastate acetate. The induced changes in the phosphatidylserine (PS) externalization and cell surface CD69 expression were followed by fluorescence-activated cell sorter and immunofluorescence.
Results:
After the induction of apoptosis PS externalizes in 52.3% of Jurkat cells. Cells cultured with Pg show tendency to a decrease of PS positive cells (42%). The opposite effect is observed in activated cells--PS externalization increase from 33.8% of control cells to 40.1% of Pg-treated cells.
Conclusions:
These findings would suggest that by increasing activation and decreasing apoptosis Pg could regulate local immune system during pregnancy.
More Related Videos
06:15Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
Published on: August 18, 2018
19:44Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen
Published on: May 30, 2012
Related Concept Videos
The Extrinsic Apoptotic Pathway
Phagocytosis of Apoptotic Cells
Normal cells contain receptors that prevent them from being recognized by phagocytes.
The Intrinsic Apoptotic Pathway
Apoptosis
Cellular Injury V: Apoptosis and Autophagy
The JAK-STAT Signaling Pathway