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Updated: Jul 20, 2026
A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
[Screening of proteins interacting with hepatitis C virus NS3 from T7-phage display library]
Ying Huang1, Xue-fei Cai, Mao-rui He
1Molecular Biology Laboratory of Infectious Diseases, Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
Objective:
To screen and identify proteins that interact with hepatitis C virus NS3 by means of T7-phage display system.
Methods:
Hepatitis C virus NS3 was expressed by prokaryotic expression and used as a selected molecule to biopan the T7 select human liver cDNA library; the selected positive clones were identified using DNA sequencing and analyzed with BLAST program in GenBank.
Results:
After BLAST analysis in all the positive clones, the proteins which interacted with the hepatitis C virus NS3 were found to be serpin peptidase inhibitor, clade A, member 1 (SERPINA1) and cyclophilin-LC.
Conclusion:
T7-phage display system is a convenient, rapid and effective method for screening interacting proteins. The proteins thus selected will provide an important means for studying the pathogenesis and carcinogenesis of HCV.
