Related Experiment Video
Updated: Jul 20, 2026

Production of IgG Fusion Proteins Transiently Expressed in Nicotiana benthamiana
Published on: January 16, 2021
Expression of single-chain antibody-barstar fusion in plants
Ekaterina G Semenyuk1, Oleg A Stremovskiy, Evelina F Edelweiss
1Branch of Shemiakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 6 Nauki Avenue, Pushchino, Moscow region 142290, Russia.
Abstract:
We successfully cloned and expressed a single-chain antibody (425scFv), that is directed to human epidermal growth factor receptor HER1 (EGFR) in transgenic tobacco plants as a fusion with bacterial barstar gene (425scFv-barstar). Plant-produced recombinant 425scFv-barstar was recovered using barstar-barnase system. Based on barstar-barnase affinity, during purification of the plant-produced 425scFv-barstar, we generated bispecific scFv-antibody heterodimers from individual single-chain fragments initially produced in different host systems with binding activity to both HER1 and HER2/neu tumor antigens. We demonstrated by flow cytometry and indirect immunofluorescent microscopy that both the components of heterodimer retain its specific cell-binding activity.
Related Concept Videos
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Tagging and Fusion Proteins
Cell Signaling in Plants

