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The Agilent in situ-synthesized microarray platform.

Paul K Wolber1, Patrick J Collins, Anne B Lucas

  • 1Microarray Quality Agilent Technologies, Inc., Santa Clara, California, USA.

Methods in Enzymology
|August 30, 2006
PubMed
Summary
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Agilent microarrays offer versatile two-color applications for gene expression profiling and comparative genomic hybridization. This guide details optimized methods and troubleshooting for these essential microarray techniques.

Area of Science:

  • Biotechnology
  • Genomics
  • Molecular Biology

Background:

  • Microarray technology is a standard laboratory tool.
  • Agilent Technologies provides catalog and custom long-oligonucleotide (60-mer) microarrays.
  • These microarrays support various two-color applications.

Purpose of the Study:

  • To outline current best practices for using Agilent microarrays.
  • To provide detailed instructions for advanced techniques.
  • To address common challenges in two-color microarray experiments.

Main Methods:

  • Focus on optimized methods for gene expression profiling.
  • Focus on optimized methods for comparative genomic hybridization.
  • Discuss evolving methods for location analysis.

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Main Results:

  • Established protocols for gene expression profiling using Agilent microarrays.
  • Established protocols for comparative genomic hybridization using Agilent microarrays.
  • Identification of solutions for common two-color microarray issues.

Conclusions:

  • Agilent's 60-mer microarrays are suitable for multiple two-color applications.
  • Optimized methods enhance the utility of microarrays for gene expression and CGH.
  • This resource aids researchers in effectively utilizing Agilent microarray technology.