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Overlapping 3q28 amplifications in the COMA cell line and undifferentiated primary sarcoma
Thomas Hussenet1, Nedjoua Mallem, Richard Redon
1Molecular Pathology Department, Institute of Genetics, Cellular and Molecular Biology, CNRS/INSERM/Collège de France, 1 rue Laurent Fries, BP10142, 67404 Illkirch Cedex, C.U. de Strasbourg, France.
Cancer Genetics and Cytogenetics
|August 30, 2006
Summary
Researchers identified amplified oncogenes on chromosome 3q in sarcoma cells. They found a 1-Mb segment at 3q28, containing specific genes, amplified in double minute chromosomes, potentially driving cancer.
Area of Science:
- Oncology
- Genetics
- Molecular Biology
Background:
- Double minute (dmin) chromosomes are known to harbor amplified oncogenes.
- The COMA cell line, derived from sarcoma, possesses DMs containing chromosome 3 material.
- Identifying specific oncogenes on 3q within DMs is crucial for understanding sarcoma development.
Purpose of the Study:
- To pinpoint putative oncogenes on chromosome 3q amplified within DMs in the COMA cell line.
- To investigate the presence and extent of 3q28 amplifications in primary sarcomas.
Main Methods:
- Microarray-based comparative genomic hybridization (array-CGH) was employed to analyze the COMA cell line.
- Fluorescence in situ hybridization (FISH) confirmed the amplification and localization of the 3q28 segment within DMs.
- Gene expression analysis (transcriptional levels) was performed on amplified regions in COMA cells versus a control cell line.
Main Results:
- A 1-Mb segment at 3q28, encompassing LPP, FLJ42393, and hsa-mir-28, was found to be amplified in the COMA cell line.
- FISH confirmed numerous copies of this 3q28 segment within DMs.
- Two poorly characterized ESTs (AI338598 and BX118304) within the amplified region showed a 20-fold increase in expression.
Conclusions:
- Amplification of the 3q28 segment, including specific genes and micro-RNA, occurs in DMs within the COMA sarcoma cell line.
- Similar amplifications were detected in a subset of primary undifferentiated sarcomas.
- The functional significance of the highly upregulated ESTs AI338598 and BX118304 in MFH carcinogenesis requires further investigation.
