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Related Concept Videos

MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
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Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
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A mitotically inheritable unit containing a MAP kinase module.

Sébastien Kicka1, Crystel Bonnet, Andrew K Sobering

  • 1Institut de Génétique et Microbiologie, Unité Mixte de Recherche 8621, Centre National de la Recherche Scientifique, Université de Paris-Sud 11, Bâtiment 400, F-91405 Orsay Cedex, France.

Proceedings of the National Academy of Sciences of the United States of America
|August 30, 2006
PubMed
Summary

This study suggests a MAP kinase cascade in Podospora anserina can self-activate, creating a prion-like hereditary unit "C" that causes cell degeneration. This expands protein-based inheritance to self-activating regulatory networks.

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Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
11:23

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Published on: June 30, 2019

Area of Science:

  • Molecular Biology
  • Genetics
  • Mycology

Background:

  • Prions are infectious proteins causing neurodegenerative diseases.
  • Prion-like hereditary units can be based on protein conformation or cleavage.
  • The filamentous fungus Podospora anserina is a model for studying non-Mendelian inheritance.

Purpose of the Study:

  • To investigate the self-activation of a MAP kinase cascade in Podospora anserina.
  • To provide evidence for a novel hereditary unit, termed "C", with prion-like properties.
  • To explore the role of MAP kinase genes in the propagation of "C".

Main Methods:

  • Genetic analysis of MAP kinase cascade genes (MAPKKK, MAPKK, MAPK).
  • Overexpression studies of key genes.
  • Microscopic analysis of MAPK localization within cells.

Main Results:

  • The MAP kinase cascade, including MAPKKK, MAPKK, and MAPK genes, is essential for the propagation of hereditary unit "C".
  • Overexpression of MAPKKK and MAPK facilitates the appearance of "C".
  • A correlation was observed between the presence of "C" and the nuclear localization of MAPK.

Conclusions:

  • The MAP kinase cascade in Podospora anserina can self-activate, generating a prion-like hereditary unit "C".
  • Unit "C" transmission shares similarities with prion propagation.
  • This expands the concept of protein-based inheritance to self-activating regulatory networks.