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Updated: Jul 20, 2026
Inguinal Subcutaneous White Adipose Tissue (ISWAT) Transplantation Model of Murine Islets
Published on: February 16, 2020
[Co-culture of cryopreserved rat islets with small intestinal submucosa]
Jun Hou1, He-li Xiang, Xin-shun Feng
1Center of Renal Transplantation, First Hospital of Xi'an Jiaotong University, Xi'an 710061, China. houjundoctor@sina.com
Objective:
To investigate the feasibility and benefits of co-culture of cryopreserved islets with small intestinal submucosa (SIS).
Methods:
Purified rat islets cryopreserved for one month were divided into SIS group and control group, and after culture in standard islet culture media RPMI1640 for 1 week, the morphology and function of the islets were assessed.
Results:
The SIS protects the fragile islets from damage by cryopreservation, and increased the recovery from (60.6-/+3.3)% to (91.7-/+1.8) % (P<0.05). Compared with the control group, incubation of the islets of the SIS group in high-glucose (16.7 mmol/L) solution resulted in significantly enhanced insulin secretion (23.7-/+1.6 vs 12.5-/+1.1 mU/L, P<0.05). When the islets were incubated in high-glucose solution containing theophylline, the calculated stimulation index of SIS group was about 3-fold higher than that of the control group.
Conclusion:
Co-culture of cryopreserved rat islets with SIS can increase the recovery of islet cells and improve their function.
