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Published on: April 29, 2017
[Labeling of CDTPA-dianhydride-coupled CD45 monoclonal antibody with yttrium-90]
Yun-bi Fu1, Gui-ping Li, Fan-yi Meng
1Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. hnfyb@yahoo.com.cn
Objective:
To explore the methods for labeling CDTPA-coupled CD45 monoclonal antibody (mAb) with yttrium-90 ((90)Y) for potential acute myeloid therapy.
Methods:
CD45 mAb was labeled with (90)Y by CDTPA and the labeling rate, radiochemical purity, final specific activity, and immunological activity of the mAb were detected.
Results:
With the optimal molar ratio of CDTPA/Ab at 20:1, the labeling rate was 95%, radiochemical purity 99.8%, and final specific activity 1.9 mCi/mg. This conjugate was stable in vitro with comparable immunological activity in comparison with unlabeled CD45 mAb.
Conclusion:
(90)Y-CDTPA-CD45 mAb possesses good properties as an ideal targetting therapeutic agent for acute leukemia.
