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Related Experiment Video

Updated: Jul 20, 2026

Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry
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Published on: July 13, 2019

Phylogenetic analysis of polyomavirus BK sequences.

Preety M Sharma1, Gaurav Gupta, Abhay Vats

  • 1Division of Transplant Pathology, E737 UPMC-Montefiore Hospital, 3459 Fifth Ave., Pittsburgh, PA 15213, USA.

Journal of Virology
|August 31, 2006
PubMed
Summary

A new whole-genome analysis reveals six BK polyomavirus (BKV) genotypes, including two novel types (V and VI). This reclassification refines understanding of BKV diversity and evolution, particularly in kidney transplant patients.

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Published on: July 27, 2018

Area of Science:

  • Virology
  • Genomics
  • Immunology

Background:

  • BK polyomavirus (BKV) is a significant pathogen in kidney transplant recipients.
  • Current BKV classification relies on limited VP1 gene sequencing, potentially underestimating diversity.

Purpose of the Study:

  • To perform a comprehensive phylogenetic analysis of BKV using whole-genome sequencing.
  • To re-evaluate and refine the taxonomic classification of BKV strains.
  • To investigate BKV diversity across various clinical settings.

Main Methods:

  • Phylogenetic analysis of whole-genome sequences from 45 BKV strains.
  • Comparative analysis of sequence variation in VP1, VP2, VP3, and large T-antigen genes.
  • Identification of polymorphic sites and type-specific amino acid changes.

Main Results:

  • Classification of BKV into six genotypes, with two newly identified types (V and VI).
  • Demonstration of heterogeneity within previously defined BKV type I strains.
  • Identification of significant sequence variation in both VP1 and large T-antigen genes, with the latter offering taxonomically informative changes.
  • Distinct subclusters observed for BKV strains from healthy individuals and those with specific immune-compromising conditions.
  • Overlapping BKV strain clusters within kidney transplant patients, irrespective of asymptomatic viruria or allograft nephropathy.

Conclusions:

  • Whole-genome phylogenetic analysis provides a more robust classification of BKV genotypes.
  • The large T-antigen gene contains valuable taxonomic information, complementing VP1 analysis.
  • BKV strain diversity and evolution are influenced by host factors, but distinct separation is not always observed within the kidney transplant population.