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In situ separation of nucleolar components by hypotonic treatment of cells
P Hozák1, O V Zatsepina, Z Likovský
1Institute of Experimental Medicine, Czechoslovak Academy of Sciences, Prague.
Biology of the Cell
|January 1, 1990
Abstract:
The main features of the reaction of nucleolar components to a simple hypotonic shock are described. In place of nucleoli, only fibrillar complexes remain. Such preservation provides another morphological proof of the hypothesis of fibrillar complexes as morphological-functional units in active nucleoli. Moreover, this method can become a good tool for further study of nucleolus-organizing regions.