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Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases
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Pharmacologic vitreolysis with microplasmin increases vitreous diffusion coefficients.

J Sebag1, Rafat R Ansari, Kwang I Suh

  • 1VMR Institute, 7677 Center Avenue, suite 400, Huntington Beach, CA 92647, USA. jsebag@VMRinstitute.com

Graefe'S Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie
|August 31, 2006
PubMed
Summary

Pharmacologic vitreolysis using microplasmin liquefies vitreous humor, enhancing vitreo-retinal surgery. This study shows microplasmin increases vitreous diffusion coefficients in a dose-dependent manner.

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Whole Vitreous Humor Dissection for Vitreodynamic Analysis
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Whole Vitreous Humor Dissection for Vitreodynamic Analysis
04:41

Whole Vitreous Humor Dissection for Vitreodynamic Analysis

Published on: May 24, 2015

Area of Science:

  • Ophthalmology
  • Biochemistry

Background:

  • Pharmacologic vitreolysis aims to improve vitreo-retinal surgery by liquefying and detaching the vitreous humor.
  • The precise mechanism of action for pharmacologic vitreolysis agents is not fully understood.
  • This study investigates microplasmin's effect on vitreous diffusion coefficients using dynamic light scattering (DLS).

Purpose of the Study:

  • To investigate the effect of microplasmin on vitreous diffusion coefficients.
  • To understand the mechanism of action for pharmacologic vitreolysis.
  • To provide insights for future clinical trials and surgical enhancement.

Main Methods:

  • Dynamic light scattering (DLS) was used to measure vitreous diffusion coefficients in 18 porcine eyes.
  • Specimens were analyzed at 37°C, 30 minutes post-injection of human recombinant microplasmin.
  • Doses ranged from 0.125 to 0.8 mg, using 20-nm tracer nanospheres.

Main Results:

  • Microplasmin significantly increased porcine vitreous diffusion coefficients.
  • The effect was dose-dependent, with an 85% increase at the maximum dose (r=0.93).
  • Findings were consistent with previous studies on bovine and human vitreous.

Conclusions:

  • Human recombinant microplasmin increases vitreous diffusion coefficients in vitro.
  • Results inform dosing, administration, and efficacy determination for pharmacologic vitreolysis.
  • Findings aid in designing clinical trials for posterior vitreous detachment induction.