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Related Experiment Videos

The complement SC5b-9 complex mediates cell adhesion through a vitronectin receptor.

G Biesecker1

  • 1Department of Pathology, Hahnemann University, Philadelphia, PA 19102.

Journal of Immunology (Baltimore, Md. : 1950)
|July 1, 1990
PubMed
Summary
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Cells attach to the serum S-protein (SC5b-9) complex via its vitronectin component interacting with cell surface vitronectin receptors. This cell adhesion mechanism is crucial for inflammation and tissue regeneration.

Area of Science:

  • Cell Biology
  • Immunology
  • Biochemistry

Background:

  • The terminal complement complex (TCC) is formed by C5b-9.
  • Serum S-protein (vitronectin) associates with the TCC, forming SC5b-9.
  • Cellular interactions with complement components are critical in inflammation and tissue repair.

Purpose of the Study:

  • To investigate cell adhesion to the SC5b-9 complex.
  • To identify the specific components and receptors involved in this interaction.
  • To explore the potential biological significance of SC5b-9 mediated cell attachment.

Main Methods:

  • Microtiter plate cell attachment assays using L8 myoblasts.
  • Testing adhesion to SC5b-9, its components, and other extracellular matrix proteins (collagen, laminin, fibronectin).

Related Experiment Videos

  • Inhibition studies using antibodies, synthetic peptides (Arg-Gly-Asp), and cation dependency analysis.

    • Main Results:

    • L8 myoblasts adhered and spread on SC5b-9 and its vitronectin/S-protein component.
    • Adhesion was specifically mediated by the vitronectin component, blocked by anti-vitronectin/S-protein antibody.
    • Attachment involved vitronectin receptors, was cation-dependent, and inhibited by Arg-Gly-Asp peptides.

    Conclusions:

    • Cell adhesion to SC5b-9 occurs through the vitronectin component binding to integrin vitronectin receptors.
    • This interaction may play a role in leukocyte migration during inflammation.
    • SC5b-9 mediated cell attachment could be important for tissue regeneration after injury.