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Updated: Jul 20, 2026
14:48
Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
Published on: May 8, 2021
Urinary proteomics
José M González-Buitrago1, Laura Ferreira, Isabel Lorenzo
1Unidad de Investigación, Hospital Universitario, Salamanca, Spain. buitrago@usal.es
Clinica Chimica Acta; International Journal of Clinical Chemistry
|September 1, 2006
Summary
Urinary proteomics analyzes urine proteins to discover disease biomarkers. This review highlights its use in diagnosing kidney diseases, transplant rejection, cancers, and kidney stones.
Area of Science:
- Clinical proteomics and biomarker discovery.
- Advancements in mass spectrometry for protein identification.
Background:
- Urinary protein measurement is a long-standing method for renal disease diagnosis and monitoring.
- Proteinuria patterns aid in identifying disease causes and classification.
- Clinical proteomics offers powerful tools for disease investigation.
Purpose of the Study:
- To review the current state of urinary proteomics.
- To emphasize the application of urinary proteomics in diagnosing specific renal and urological conditions.
- To highlight the identification of disease-specific protein biomarkers in urine.
Main Methods:
- Utilizes protein separation techniques.
- Employs mass spectrometry for protein identification.
- Compares normal urinary proteomes with those from diseased patients to identify differential protein expression.
Main Results:
- Proteomics enables the identification of novel urinary biomarkers.
- Differential protein expression patterns can distinguish between healthy and diseased states.
- Established the foundation for using urinary proteome as a diagnostic tool.
Conclusions:
- Urinary proteomics is a valuable tool for diagnosing glomerular diseases.
- It aids in the detection of renal allograft rejection.
- Applications extend to diagnosing urological cancers and urolithiasis.
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