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Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
Published on: May 19, 2023
Mitochondrial function in vivo evaluated by NADH fluorescence: from animal models to human studies
Avraham Mayevsky1, Gennady G Rogatsky
1The Mina & Everard Goodman Faculty of Life Sciences and The Leslie and Susan Gonda Multidisciplinary Brain Research Center, Bar-Ilan Univ., Ramat-Gan 52900, Israel. mayevsa@mail.biu.ac.il
American Journal of Physiology. Cell Physiology
|September 1, 2006
Summary
Monitoring mitochondrial function via NADH fluorescence provides crucial insights into cellular health and disease. This technique aids in understanding metabolic states for improved diagnostics and treatments in both research and clinical settings.
Area of Science:
- Biomedical Engineering
- Cellular Metabolism
- Mitochondrial Physiology
Background:
- Normal mitochondrial function is essential for cellular homeostasis.
- Mitochondrial dysfunction is implicated in numerous pathological conditions.
- Real-time in vivo monitoring of mitochondrial metabolic state is vital.
Purpose of the Study:
- To review the history and physiological significance of NADH fluorescence monitoring.
- To present the scientific basis of NADH fluorescence measurements.
- To detail methodologies, current fluorometers, and factors affecting NADH signals.
Main Methods:
- Review of historical and current literature on NADH fluorescence monitoring.
- Analysis of methodologies for in vivo tissue monitoring.
- Discussion of fluorometer technology and signal interpretation.
Main Results:
- NADH fluorescence monitoring offers valuable data on mitochondrial energy production and intracellular oxygen levels.
- Trend monitoring of NADH signals is crucial for interpreting physiological and pathological states.
- Multiparametric approaches, with NADH as a key parameter, enhance tissue function understanding.
Conclusions:
- NADH fluorescence monitoring is a critical tool for assessing mitochondrial metabolic state in vivo.
- Advancements in technology enable clinical applications for improved patient diagnosis.
- Understanding NADH dynamics is key to interpreting cellular energy metabolism and disease states.
