Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Calvin Cycle01:40

The Calvin Cycle

OverviewOxygenic photosynthesis plays a central role in the global carbon and oxygen cycles. The carbohydrates produced support nearly all food webs, while the oxygen by‑product enables aerobic life.Light‑dependent and light‑independent reactionsPhotosynthesis occurs in two main stages, each in a different part of the chloroplast: light‑dependent reactions and light‑independent reactions, also called the Calvin‑Benson cycle or simply the Calvin cycle.Light‑dependent reactions take place in the...
Protein Transport to the Stroma01:24

Protein Transport to the Stroma

Chloroplasts are triple membrane structures with an outer membrane, an inner membrane, and a thylakoid membrane, each containing distinct metabolite transporters, membrane translocons, and enzymes. Appropriate sorting and translocating these proteins to their correct membrane systems is essential for chloroplast function.
Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
Biosynthesis in Bacteria01:24

Biosynthesis in Bacteria

Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
Amino Acid Biosynthetic Pathways01:29

Amino Acid Biosynthetic Pathways

Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which provide...
Biosynthesis of Nucleic Acids01:28

Biosynthesis of Nucleic Acids

Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
Biosynthesis of Lipids01:29

Biosynthesis of Lipids

Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis pathway, which...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural bioinformatic studies of eight integral transmembrane NADPH oxidases and their AlphaFold 3 predicted QTY analogs with reduced hydrophobicity.

PloS one·2026
Same author

Integrative Structural Bioinformatics and Molecular Dynamics Analyses of Synaptic Vesicle Proteins and Their Water-Soluble QTY-Variants Reveal Membrane Constraints and Evolutionary Coupling of T ⇔ V.

Journal of molecular evolution·2026
Same author

Deep Learning Approach for Species Identification of Forensically Important <i>Sarcophagid flies</i> (Diptera: Sarcophagidae) in China.

Insects·2026
Same author

Stretchable Organic Electrochemical Transistors with High Transconductance for Bioelectronic and Neuromorphic Applications.

ACS applied materials & interfaces·2026
Same author

AI-assisted academic writing in medical postgraduate education: a cross-sectional study of L2 challenges, affective barriers, and instructional implications.

BMC medical education·2026
Same author

Identification of PANoptosis-Related Biomarkers in Hypertrophic Cardiomyopathy: Insights from Multi-Omics Analysis.

Journal of inflammation research·2026
Same journal

Infection by Different Clades of Candidozyma auris in a Galleria mellonella Model: Determining Virulence Levels.

Mycopathologia·2026
Same journal

In Vitro Antifungal Activity of Octenidine Against Emerging Dermatophyte Species.

Mycopathologia·2026
Same journal

Candidemia Epidemiology and Antifungal Resistance in Critically-ill Patients During and After COVID-19 Pandemic: Comparison Between Two Hospitals in Greece and Türkiye.

Mycopathologia·2026
Same journal

Long-Read Sequencing and Comparative Genome Analyses of Seven Emergomyces Type-Strains.

Mycopathologia·2026
Same journal

Refractory Dermatophytosis Caused by Trichophyton indotineae: A Case Series Highlighting Antifungal Resistance and Management Challenges.

Mycopathologia·2026
Same journal

Predominance of Fonsecaea nubica in Chromoblastomycosis: A Single-Centre Study from Southern India.

Mycopathologia·2026
See all related articles

Related Experiment Video

Updated: Jun 25, 2026

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
08:56

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

Biosynthesis of dothistromin.

Rosie E Bradshaw1, Shuguang Zhang

  • 1National Centre for Advanced Bio-Protection Technologies, Institute of Molecular BioSciences, Massey University, Palmerston North, New Zealand. R.E.Bradshaw@massey.ac.nz

Mycopathologia
|September 1, 2006
PubMed
Summary
This summary is machine-generated.

Dothistromin, a mycotoxin similar to aflatoxin precursors, is produced by Dothistroma fungi during pine needle blight. Its biosynthesis involves genes similar to aflatoxin pathways, suggesting a role in early fungal invasion.

More Related Videos

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
09:50

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Published on: August 14, 2019

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

Related Experiment Videos

Last Updated: Jun 25, 2026

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
08:56

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
09:50

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Published on: August 14, 2019

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

Area of Science:

  • Mycology
  • Plant Pathology
  • Biochemistry

Background:

  • Dothistromin is a mycotoxin structurally similar to versicolorin B, a precursor for aflatoxin and sterigmatocystin.
  • Pathogenic Dothistroma species produce dothistromin in pine needles during needle blight and secrete it in culture.

Purpose of the Study:

  • To investigate the biosynthesis and genomic organization of dothistromin.
  • To compare dothistromin biosynthesis with that of aflatoxin and sterigmatocystin.

Main Methods:

  • Identification of dothistromin biosynthetic genes through sequence similarity to aflatoxin and sterigmatocystin genes.
  • Analysis of gene clustering and location on a minichromosome in Dothistroma septosporum.
  • Expression analysis of dothistromin genes during fungal growth.

Main Results:

  • Genes homologous to aflatoxin and sterigmatocystin biosynthetic genes are required for dothistromin production.
  • A unique epoxide hydrolase gene is clustered with dothistromin genes.
  • Dothistromin genes are located on a minichromosome and expressed early in fungal growth.

Conclusions:

  • Dothistromin biosynthesis shares genetic elements with aflatoxin and sterigmatocystin pathways.
  • The early expression of dothistromin genes suggests a role in the initial stages of plant infection.
  • Genomic organization and expression patterns offer insights into fungal pathogenesis and mycotoxin evolution.