Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endotoxin removal by anion-exchange polymeric matrix.

K C Hou1, R Zaniewski

  • 1CUNO, Inc., Life Sciences Division, Meriden, Connecticut 06450.

Biotechnology and Applied Biochemistry
|June 1, 1990
PubMed
Summary

Anion-exchange matrices effectively remove endotoxins from protein solutions, especially when pH and salt concentrations are controlled. Optimizing functional groups and pH range enhances endotoxin reduction capacity in biological solutions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Activity of the new fluoroquinolone trovafloxacin (CP-99,219) against DNA gyrase and topoisomerase IV mutants of Streptococcus pneumoniae selected in vitro.

Antimicrobial agents and chemotherapy·1996
Same author

Extracorporeal techniques of endotoxin removal: a review of the art and science.

Advances in renal replacement therapy·1995
Same author

Adsorption of hormones from serum by carbon embedded depth filters.

Journal of parenteral science and technology : a publication of the Parenteral Drug Association·1991
Same author

Protein A immobilized affinity cartridge for immunoglobulin purification.

Biotechnology and applied biochemistry·1991
Same author

The effect of hydrophobic interaction on endotoxin adsorption by polymeric affinity matrix.

Biochimica et biophysica acta·1991
Same author

A method for extracorporeal heparin removal from blood by affinity chromatography.

Artificial organs·1990

Area of Science:

  • Biochemistry
  • Materials Science
  • Separation Science

Background:

  • Endotoxins pose a significant challenge in purifying biological solutions.
  • Protein solutions, such as albumin and gamma-globulin, require effective endotoxin removal for safety and efficacy.
  • Anion-exchange chromatography is a potential method for endotoxin mitigation.

Purpose of the Study:

  • To evaluate the efficacy of anion-exchange polymeric matrices for endotoxin removal from albumin and gamma-globulin solutions.
  • To investigate the influence of pH, salt concentration, and matrix properties on endotoxin removal efficiency.
  • To assess the performance of these matrices under both static and dynamic conditions.

Main Methods:

  • Utilized positively charged cellulose acrylic media with DEAE or QAE functional groups.

Related Experiment Videos

  • Performed static evaluations in test tubes to assess endotoxin removal from protein solutions.
  • Conducted dynamic flow experiments using a DEAE cartridge for endotoxin reduction in albumin solutions.
  • Varied pH levels and salt concentrations to determine optimal removal conditions.

    • Main Results:

    • Anion-exchange matrices showed significant endotoxin removal from tap water but were less effective in protein solutions.
    • Controlled pH and salt concentrations improved endotoxin reduction, though very low levels (<100 pg/ml) were challenging.
    • Endotoxin removal capacity correlated with the number of functional groups (meq) and the matrix's pH operable range (pK alpha).
    • Dynamic flow experiments achieved greater than 75% endotoxin reduction in albumin solutions with minimal protein loss.

    Conclusions:

    • Anion-exchange polymeric matrices can effectively reduce endotoxin levels in biological solutions.
    • Optimizing process parameters like pH and salt concentration is crucial for efficient endotoxin removal.
    • These methods are valuable for purifying biological products derived from natural sources or recombinant DNA technology.