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Updated: Jul 20, 2026
Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
Published on: January 22, 2014
Establishment of cell free conversion system with biotin-labelled recombinant PrPsen expressed in E. coli
Jin Zhang1, Jian-Mei Gao, Feng Li
1School of Medicine, Xi'an Jiaotong University, China.
Objective:
To report a protocol using biotin-labelled PrP protein in cell free conversion assay instead of isotope.
Methods:
A hamster PrP protein (HaPrP) was expressed in E. coli and purified with HIS-tag affinity chromatograph. After being labelled with biotin, HaPrP was mixed with PrPSc preparation from scrapie strain 263K.
Results:
Protease-resistant bands were detected after four-day incubation.
Conclusion:
The new conversion model provides a reliable, easily handling, and environment-friendly method for studies of prion and transmissible spongiform encephalopathies.
