Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Dialysis

Dialysis

Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Practical quantification of immunohistochemistry antigen concentrations and reaction-diffusion parameters.

bioRxiv : the preprint server for biology·2026

Same author

A Fit-for-purpose Strategy for Clinical Immunogenicity Assessment of Multivalent Bispecific Antibodies.

The AAPS journal·2025

Same author

Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations.

EBioMedicine·2025

Same author

HPK1 citron homology domain regulates phosphorylation of SLP76 and modulates kinase domain interaction dynamics.

Nature communications·2024

Same author

Application of N-Terminal Site-Specific Biotin and Digoxigenin Conjugates to Clinical Anti-drug Antibody Assay Development.

Bioconjugate chemistry·2023

Same author

3D Printed Customizable Microsampling Devices for Neuroscience Applications.

ACS chemical neuroscience·2023

Search research articles

Related Experiment Video

Updated: Jul 20, 2026

In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes

In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes

Published on: September 26, 2018

Microdialysis sampling membrane performance during in vitro macromolecule collection.

Xiangdan Wang¹, Julie A Stenken

¹Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, 4215 Biotech Building, Rensselaer Polytechnic Institute, 110 Eighth Street, Troy, NY 12180-3590, USA.

Analytical Chemistry

|September 2, 2006

Summary

Microdialysis sampling can now collect large molecules like FITC-dextrans. Membrane resistance, due to hindered diffusion, impacts mass transport for macromolecules during sampling.

More Related Videos

Custom-made Microdialysis Probe Design

Custom-made Microdialysis Probe Design

Published on: July 21, 2015

Related Experiment Videos

Last Updated: Jul 20, 2026

In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes

In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes

Published on: September 26, 2018

Custom-made Microdialysis Probe Design

Custom-made Microdialysis Probe Design

Published on: July 21, 2015

Area of Science:

Biomedical Engineering
Analytical Chemistry
Pharmacokinetics

Background:

Microdialysis sampling is established for small molecules.
Growing interest in microdialysis for macromolecule analysis.
Challenges exist in understanding macromolecule transport.

Purpose of the Study:

Investigate mass transport of macromolecules using microdialysis.
Evaluate parameters affecting macromolecule sampling.
Assess the applicability of existing mass transfer models.

Main Methods:

Used fluorescein isothiocyanate-labeled dextrans (FITC-dextrans) (10-70 kDa).
Employed polyethersulfone membranes (100-kDa MWCO, 2 and 10 mm lengths).
Conducted experiments in buffered saline and agar solutions.

Main Results:

Analyzed analyte mass transport properties for FITC-dextrans.
Evaluated effective membrane diffusion coefficients.
Compared experimental data with the Bungay et al. mass transfer model.

Conclusions:

Membrane resistance significantly impacts larger macromolecule transport.
Hindered diffusion is a likely cause of mass transport resistance.
Presents critical experimental aspects for macromolecule microdialysis.