Related Experiment Video
Updated: Apr 3, 2026
Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes
Published on: November 26, 2008
An integrated proteomic workflow for two-dimensional differential gel electrophoresis and robotic spot picking
Ryan C Mahnke1, Todd H Corzett, Sandra L McCutchen-Maloney
1New Technologies Engineering Division, Biosciences Directorate, Lawrence Livermore National Laboratory, 7000 East Avenue, L-452, Livermore, California 94551, USA.
Abstract:
New technologies have advanced the field of proteomics, and a number of companies have developed innovative platforms to drive this research. However, significant challenges are often encountered when trying to integrate complementary technologies from multiple manufacturers. We have developed a software and hardware solution to integrate the Ettan two-dimensional difference gel electrophoresis (2-D DIGE) system (GE Healthcare) with the Investigator ProPic spot picking robot (Genomic Solutions). We have analyzed protein sample preparations from bacterial and mammalian sources to demonstrate a new workflow with increased throughput for gel-based proteomics.
More Related Videos
Related Concept Videos
01:27SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
01:22Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...