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Focused proteomics in post-mortem human spinal cord.

Titti Ekegren1, Jörg Hanrieder, Sten-Magnus Aquilonius

  • 1Department of Physical and Analytical Chemistry, Analytical Chemistry, Uppsala University, Uppsala, Sweden.

Journal of Proteome Research
|September 2, 2006
PubMed
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Researchers identified proteins in spinal cord samples from amyotrophic lateral sclerosis (ALS) patients and controls using advanced mass spectrometry. Glial fibrillary acidic protein (GFAP) was the most abundant protein in both groups.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Proteomics

Background:

  • Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease affecting nerve cells in the brain and spinal cord.
  • Understanding the molecular changes in the spinal cord is crucial for ALS research.

Purpose of the Study:

  • To identify and compare proteins in the spinal cord of ALS patients and control subjects.
  • To investigate potential proteomic biomarkers for ALS.

Main Methods:

  • Proteomic analysis using highly sensitive electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR MS).
  • Laser microdissection in combination with pressure catapulting (LMPC) for sample dissection.
  • Confirmation and further identification using nanoLC-matrix assisted laser desorption ionization time-of-flight tandem mass spectrometry (MALDI-TOF-TOF MS).

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Main Results:

  • Identified 18 significantly abundant proteins in ALS spinal cord samples and 16 in control samples (p < 0.05).
  • Glial fibrillary acidic protein (GFAP) was the most abundant protein in both groups.
  • Other identified proteins include hemoglobin chains, myelin basic protein, thioredoxin, alpha enolase, and choline acetyltransferase.

Conclusions:

  • Proteomic profiling reveals specific protein alterations in the spinal cord of ALS patients.
  • GFAP is a prominent protein in the central nervous system and is highly abundant in both ALS and control spinal cords.
  • The study provides a foundation for further investigation into the role of these proteins in ALS pathogenesis.