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Related Experiment Videos

Intermediate trophoblast: further immunocytochemical characterization.

I T Yeh1, D M O'Connor, R J Kurman

  • 1Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia.

Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc
|May 1, 1990
PubMed
Summary

Keratin and epithelial membrane antigen (EMA) antibodies are more sensitive markers for identifying intermediate trophoblast cells than human placental lactogen (hPL). These markers aid in distinguishing intermediate trophoblast from decidual cells during gestation.

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Area of Science:

  • Reproductive biology
  • Cell biology
  • Histology

Background:

  • Intermediate trophoblast is a recently identified subpopulation of trophoblast.
  • Previous studies used human placental lactogen (hPL) and beta-human chorionic gonadotropin (beta-hCG) for identification, with inconsistent results.

Purpose of the Study:

  • To further characterize intermediate trophoblast immunocytochemical features.
  • To identify more sensitive markers for intermediate trophoblast identification.
  • To differentiate intermediate trophoblast from decidual cells.

Main Methods:

  • Studied 88 placentas and implantation sites (4-40 wk gestation).
  • Utilized a panel of antibodies: keratin, epithelial membrane antigen (EMA), placental alkaline phosphatase (PLAP), alpha-hCG, and prolactin.

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  • Compared antibody reactivity with known markers like hPL and distinguished from decidual cells.

    • Main Results:

    • Keratin was consistently expressed in intermediate trophoblast throughout gestation.
    • EMA showed expression in the second and third trimesters, but less consistently than keratin.
    • Keratin and EMA identified both hPL-positive and hPL-negative intermediate trophoblast cells.
    • Alpha-hCG reactivity was observed in the first and second trimesters.
    • PLAP and prolactin showed minimal reactivity.
    • Decidual cells did not react with any tested antibodies.

    Conclusions:

    • Antibodies against keratin and EMA are more sensitive markers for intermediate trophoblast than hPL.
    • Keratin and EMA are valuable for distinguishing intermediate trophoblast from decidual cells.