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Updated: Jun 30, 2026
Isolation of Leukocytes from the Human Maternal-fetal Interface
Published on: May 21, 2015
[The expression of SDF-1/CXCR4 in trophoblasts and their role in maternofetal immune tolerance]
Yun Yang1, Li Zou, Yi-ying Wang
1Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Aim:
To investigate the expression of SDF-1/CXCR4 in trophoblasts and their role in maternofetal immune tolerance.
Methods:
Extra-villous trophoblasts were highly purified from villi of first trimester. The expression of SDF-1 and CXCR4 in villi was detected by immunocytochemical staining. A chorioepithelioma cell line from trophoblasts with high expression of CXCR4 was screened by flow cytometry to test the chemotaxis of SDF-1 in transwell. The expression of SDF-1 and CXCR4 in villi of first trimester and placentas of last trimester was detected by immunohistochemical staining.
Results:
SDF-1 and CXCR4 were expressed in extra villous trophoblasts. In a certain coverage, the chemotactic activity of SDF-1 had positive correlation with its concentration (r=0.68, P<0.01), and the maximum CI (chemotactic index) was 1.62+/-0.12.
Conclusion:
SDF-1 and CXCR4 play an important role in gestation and they are also important in maternofetal immune tolerance during pregnancy.
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