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Published on: October 20, 2021
[Expression map of immune-related genes on PBMCs in familial clustering patients with chronic virus hepatitis B]
Ying-li He1, Ying-ren Zhao, Shu-lin Zhang
1Infectious Department, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China. heyingli2000@163.com
Aim:
To investigate peripheral blood mononuclear cells (PBMCs) immune-related gene expression profile in patients with chronic virus hepatitis B(CHB) by oligonucleotide gene array technique.
Methods:
PBMCs were collected from the members of a family of clustering hepatitis B virus (HBV) infection including 5 CHB patients and 4 healthy spouses and RNA prepared from PBMCs was hybridized to high-density oligonucleotide arrays(HG-U133A 2.0 Human Gene Chips, Affymetrix), covering the expression of 22 000 human ESTs. Primary scanned image was analyzed with DNT software package.
Results:
Out of the 22 000 ESTs, 24 different immune-related genes were identified. Among the 24 genes, 7 genes showed increased expression and 17 genes showed decreased expression in CHB compared with those in healthy spouses. The up-regulated genes were mainly associated with adaptive immunity, while the down-regulated genes were associated with innate immunity.
Conclusion:
Our findings suggest that HBV infection alters a broad range of immunity genes expression and innate immunity-associated genes are important in the defense against HBV chronic infection.
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