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Published on: January 16, 2019
[Determination of rhodamine 123 in cell lysate by high-performance liquid chromatography with visible wavelength
1Department of Pharmacy, Hunan Tumor Hospital, Changsha 410006, China. sjping333@126.com
Objective:
To establish a high-performance liquid chromatography with visible wavelength detection method for rhodamine 123 in cell lysate.
Methods:
The HPLC separation was performed on a Kromasil C(18) (5 microm, 4.6 mm x 250 mm) column, using water (15 mmol/L potassium acetate and 1.2 mmol/L tetrabutylammonium bromide) - acetonitrile (65:35) as the mobile phase. The wave length was 390 nm, the internal standard was rhodamine B. Protein in the sample was precipitated by trichloroacetic acid.
Results:
The calibration curve was linear in the range of 3 - 300 microg/L. The intra-day and inter-day RSDs were less than 5%.
Conclusion:
The method is accurate,sensitive, simple, and reliable for determining rhodamine 123 in cell lysate.
