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A Timeless debate: resolving TIM's noncircadian roles with possible clock function.

Anthony L Gotter1

  • 1Division of Human Genetics and Molecular Biology, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA. gotter@email.chop.edu

Neuroreport
|September 5, 2006
PubMed
Summary
This summary is machine-generated.

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Mammalian TIMELESS (TIM) is evolutionarily distinct from insect circadian TIM proteins. Its vital cellular roles complicate determining its specific function in the mammalian circadian clock.

Area of Science:

  • Chronobiology
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Mammalian TIMELESS (TIM) was initially identified based on sequence similarity to Drosophila TIM, a key circadian clock protein.
  • Existing research presents conflicting findings on mammalian TIM's rhythmic expression, protein interactions, and role in clock gene transcription.

Purpose of the Study:

  • To clarify the evolutionary relationship of mammalian TIM to other TIM family members.
  • To investigate the potential role of mammalian TIM within the circadian clock mechanism.

Main Methods:

  • Comprehensive sequence analysis of mammalian TIM.
  • Comparison with Drosophila TIM and TIM-like sequences (TIMEOUT).
  • Analysis of evolutionary conservation within the TIM ortholog family.

Related Experiment Videos

Main Results:

  • Mammalian TIM shows greater sequence similarity to Drosophila TIMOUT than to insect circadian TIM.
  • Mammalian TIM belongs to an evolutionarily conserved family of TIM orthologs, separate from insect-specific circadian TIM proteins.

Conclusions:

  • Mammalian TIM is evolutionarily distinct from insect circadian TIM proteins.
  • The essential, conserved cellular functions of mammalian TIM orthologs obscure its specific role in the circadian clock.