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Updated: Jul 20, 2026
09:32
A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
Published on: September 23, 2014
Tissue microarrays as a platform for proteomic investigation.
Joon-Yong Chung1, Till Braunschweig, Kimberly Tuttle
1Tissue Array Research Program, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, MSC 4605, Bethesda, MD 20892-4605, USA.
Journal of Molecular Histology
|September 6, 2006
Summary
Tissue microarrays are vital in pathology for confirming experimental results and exploring protein expression. New proteomic approaches enhance their utility in basic science and clinical research for biomarker discovery.
Area of Science:
- Pathology
- Proteomics
- Biomarker Discovery
Background:
- Tissue microarrays are essential in translational pathology.
- They validate findings from other experimental platforms like expression microarrays.
- Used for exploring protein expression profiles via immunohistochemical analysis.
Purpose of the Study:
- To explore new approaches applied to tissue microarrays.
- To characterize the human proteome using tissue microarrays.
- To highlight new technologies enabling tissue microarrays to function as protein arrays.
Main Methods:
- Application of traditional protein-based assays.
- Integration of novel assays onto the tissue microarray platform.
- Utilizing tissue microarrays for proteomic analysis.
Main Results:
- Tissue microarrays confirm results from expression microarrays.
- They are routinely used in molecular epidemiology and drug development.
- Tissue microarrays are pivotal in determining the value of new biomarkers.
Conclusions:
- Tissue microarrays have gained new utility as a proteomic tool.
- Novel approaches enhance their role in basic science and clinical investigation.
- These advancements facilitate comprehensive human proteome characterization.
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