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Calcium-dependent histidine and histamine release from superfused synaptosomes
T Tikkanen1, O Raatikainen, L Tuomisto
1Department of Pharmacology and Toxicology, University of Kuopio, Finland.
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|April 1, 1990
Summary
Calcium is crucial for the release of both histidine and histamine from guinea pig synaptosomes. This study confirms calcium-dependent release mechanisms for these neurotransmitters.
Area of Science:
- Neuroscience
- Neurochemistry
Background:
- Histamine and histidine are neurotransmitters involved in various brain functions.
- Synaptosomes are isolated nerve terminals used to study neurotransmitter release.
Purpose of the Study:
- To investigate the role of calcium in the release of histidine and histamine from guinea pig synaptosomes.
- To confirm calcium-dependency of histamine release in a superfusion system.
Main Methods:
- Superfusion system using guinea pig synaptosomes preloaded with 3H-histidine.
- Depolarization induced by potassium (K+) to stimulate neurotransmitter release.
- Calcium removal to assess its effect on release.
Main Results:
- Potassium-induced depolarization caused simultaneous release of histidine and histamine.
- Removal of calcium significantly diminished the release of both substances.
- Calcium-dependency of histamine release was confirmed in synaptosomes.
- Potassium-induced histidine release was found to be at least partially calcium-dependent.
Conclusions:
- Calcium ions play a significant role in the release of both histidine and histamine from synaptosomes.
- The findings support a calcium-dependent mechanism for neurotransmitter release in neuronal preparations.