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Related Experiment Videos

Differentiation of PC12 cells.

Elizabeth M Adler1, Nancy R Gough, Jay A Blundon

  • 1American Association for the Advancement of Science, 1200 New York Avenue, N.W., Washington, DC 20005, USA. eadler@aaas.org

Science'S STKE : Signal Transduction Knowledge Environment
|September 7, 2006
PubMed
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This laboratory exercise uses PC12 cells to investigate cellular differentiation signaling pathways. Advanced undergraduates in neurobiology and cell biology can learn essential lab techniques and concepts.

Area of Science:

  • Cell Biology
  • Neurobiology
  • Biochemistry

Background:

  • Cellular differentiation is a fundamental process in development and disease.
  • Understanding signaling pathways is crucial for studying cell fate determination.
  • PC12 cells are a well-established model system for neuronal differentiation.

Purpose of the Study:

  • To provide advanced undergraduate students with hands-on experience in studying cellular differentiation.
  • To explore the signaling pathways regulating differentiation using a cultured cell system.
  • To develop practical laboratory skills in cell biology and neurobiology.

Main Methods:

  • Utilizing PC12 cells as a model system for neuronal differentiation.
  • Designing and conducting a series of collaborative laboratory exercises over three weeks.

Related Experiment Videos

  • Investigating specific signaling pathways implicated in the differentiation process.

    • Main Results:

    • Students gain practical experience in cell culture and experimental design.
    • Students develop an understanding of signaling pathways controlling cellular differentiation.
    • The laboratory exercises facilitate the assessment of student comprehension and performance.

    Conclusions:

    • This laboratory curriculum effectively integrates theoretical knowledge with practical application in cell signaling and differentiation.
    • The use of PC12 cells provides a robust platform for teaching advanced concepts in neurobiology and cell biology.
    • The provided guidelines support effective student learning and performance evaluation in a laboratory setting.