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Robust 3D DNA FISH Using Directly Labeled Probes
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Robust 3D DNA FISH Using Directly Labeled Probes

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Towards many colors in FISH on 3D-preserved interphase nuclei

J Walter1, B Joffe, A Bolzer

  • 1Chair of Anthropology and Human Genetics, Department of Biology II, Ludwig-Maximilians-University, Martinsried, Germany.

Cytogenetic and Genome Research
|September 7, 2006
PubMed

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...