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Published on: March 5, 2019
[A morphological study of chondrogenesis by adipose-derived adult stem cells induced by recombinant human bone
Yiyong Wei1, Yunyu Hu, Wei Hao
1Institute of Orthopedics, Xijing Hospital, Fourth Military University, Xi'an Shaanxi, 710032, PR China.
Objective:
To investigate the possibility of differentiation of the isolated and cultured adipose derived adult stem cells into chondrocytes, which is induced by the recombinant human bone morphogenetic protein 2 (rhBMP 2).
Methods:
The rabbit adipose tissue was minced and digested by collagenase Type I. The adipose derived adult stem cells were obtained and then they were cultured in the micropellet condition respectively in the rhBMP-2 group, the rhTGF-beta1 group, the combination group, and the control group for 14 days. The differentiation of the adipose-derived stem cells into chondrocytes was identified by the histological methods including HE, Alcian blue, Von kossa, and immunohistochemical stainings.
Results:
After the continuous induction by rhBMP-2 and continuous culture for 14 days, the HE staining revealed a formation of the cartilage lacuna; Alcian blue indicated that proteoglycan existed in the extracellular matrix; the immunohistochemical staining indicated that collagen II was in the cellular matrix; and Von kossa indicated that the adipose-derived stem cells could not differentiate into the osteoblasts by an induction of rhBMP-2.
Conclusion:
In the micropellet condition, the adipose-derived adult stem cells can differentiate into the chondrocytes, which is initially induced by rhBMP-2. This differentiation can provide a foundation for the repair of the cartilage injury.
