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Updated: Jul 20, 2026

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
Hymenobacter rigui sp. nov., isolated from wetland freshwater
Keun Sik Baik1, Chi Nam Seong1, Eun Young Moon2
1Department of Biology, College of Natural Sciences, Sunchon National University, Sunchon 540-742, Republic of Korea.
Abstract:
A non-motile, rod-shaped and pinkish-red-pigmented bacterium, designated strain WPCB131(T), was isolated from freshwater of Woopo wetland, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that this isolate represents a novel member of the genus Hymenobacter with sequence similarities of 93.2-95.5 % to known Hymenobacter species. No other taxa in the phylum Bacteroidetes showed more than 90 % sequence similarity to the isolate. Cells were Gram-negative, oxidase-negative and catalase-positive. Optimum growth was observed at pH 6 and 30 degrees C. The major fatty acids were iso-C(15 : 0) (34.8 %), C(16 : 1)omega5c (15.0 %), anteiso-C(17 : 1) B/iso-C(17 : 1) I (14.4 %) and C(16 : 1)omega7c/iso-C(15 : 0) 2-OH (13.8 %). The DNA G+C content of strain WPCB131(T) was 65 mol%. The isolate could be readily distinguished from other Hymenobacter species using a combination of phenotypic characters. On the basis of the polyphasic evidence presented in this study, it is proposed that strain WPCB131(T) (=IMSNU 14116(T)=KCTC 12533(T)=NBRC 101118(T)) represents a novel species, Hymenobacter rigui sp. nov.
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