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Related Experiment Videos

The cDNA sequencing project.

Hideko Urushihara1, Takahiro Morio, Yoshimasa Tanaka

  • 1Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki-ken, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|September 8, 2006
PubMed
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The Dictyostelium discoideum cDNA sequencing project generated over 150,000 expressed sequence tags (ESTs), aiding the study of its multicellular development.

Area of Science:

  • Molecular Biology
  • Genomics
  • Developmental Biology

Background:

  • The Dictyostelium discoideum cDNA sequencing project initiated in 1995.
  • This project preceded the genome sequencing effort for this organism.

Purpose of the Study:

  • To generate a comprehensive set of expressed sequence tags (ESTs) from Dictyostelium discoideum.
  • To facilitate research into the genetic control of multicellular development.

Main Methods:

  • Construction of 14 cDNA libraries from various developmental stages.
  • Sequencing of nearly 100,000 randomly selected clones.
  • Assembly of expressed sequence tag reads into independent genes.

Main Results:

  • Over 150,000 expressed sequence tags (ESTs) were generated.

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  • EST data were assembled into 6700 independent genes, representing approximately 55% of the estimated Dictyostelium genes.
  • Data were made publicly available online for collaboration and microarray analysis.

    • Conclusions:

    • The extensive EST data provide a valuable resource for understanding Dictyostelium discoideum.
    • This project has significantly contributed to deciphering the genetic system controlling multicellular development in Dictyostelium.