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Advances in the analysis of conformational transitions in peptides using differential scanning calorimetry
Werner W Streicher1, George I Makhatadze
1Department of Biochemistry and Molecular Biology, Penn State College of Medicine, Hershey, PA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|September 8, 2006
Abstract:
Differential scanning calorimetry can measure the heat capacity of a protein/peptide solution over a range of temperatures at constant pressure, which is used to determine the enthalpy function of the system. There are several experimental factors that can have a significant impact on the determined enthalpy and subsequent derived thermodynamic parameters. These factors are discussed in terms of sample and instrument preparation, as well as data collection and analysis.
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