Proteomic technique used in studying ovule development of Chinese pine (Pinus tabulaeformis Carr.)

Chinese pine (Pinus tabulaeformis Carr.) is one of the most important afforestation and ornamental species in China. Ovule abortion is a serious problem influencing the output of seeds and reproduction of Chinese pine. So it is of much significance to study the mechanisms of ovule development and abortion of Chinese pine. By combining two-dimensional gel electrophoresis (2-DE) and mass spectrometry analysis, the patterns of gene expression in a specific tissue at a specific stage can be displayed and characterized. This study acquired an appropriate protein extraction method from ovules of Chinese pine and optimized the conditions for protein identification by mass spectrometry.