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Updated: Jul 20, 2026
Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring
Published on: December 9, 2010
1H NMR spectroscopic imaging of the mouse brain at 9.4 T
Naoyuki Miyasaka1, Kan Takahashi, Hoby P Hetherington
1Department of Radiology, Albert Einstein College of Medicine, Bronx, New York 10461, USA. n.miyasaka.gyne@tmd.ac.jp
Purpose:
To assess the feasibility of 1H spectroscopic imaging (SI) in the mouse brain at 9.4 T, and investigate regional variations in brain metabolites.
Materials And Methods:
A total of 21 SI studies were performed in CD-1 mice to evaluate the basal ganglia (N=5), hippocampus and thalamus (N=11), and cerebellum (N=5). We adjusted the B0 homogeneity for each slice using a fully automated shim calculation method based on the B0 map, which we measured using a multislice gradient-echo sequence with multiple phase evolution delays. The SI employed a modified localization by adiabatic selective refocusing (LASER) sequence with TE/TR of 50/2000 msec, 24x24 encodes over a field of view (FOV) of 24 mmx24 mm, 1 microL voxel resolution, and two averages, for a total acquisition time of 38 minutes.
Results:
Sufficient shimming was achieved and high-quality spectra were consistently obtained in each slice. N-acetyl aspartate (NAA)/creatine (Cr) ratios in the basal ganglia and thalamus (0.86+/-0.07, and 0.87+/-0.07, respectively) were significantly higher than those in the hippocampus and cerebellum (0.76+/-0.03 and 0.67+/-0.07), which were also significantly different from each other.
Conclusion:
1H SI of the mouse brain is highly reproducible and allows differences in regional metabolite ratios to be easily visualized.
