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Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis
Published on: March 12, 2021
Batch foam separation of a soluble protein.
Hideo Maruyama1, Hideshi Seki, Akira Suzuki
1Division of Marine Biosciences, Graduate School of Fisheries Sciences, Hokkaido University, Minato 3-1-1, Hakodate 041-8611, Japan. maruyama@elsie.fish.hokudai.ac.jp
Water Research
|September 9, 2006
Summary
Foam separation effectively removed ovalbumin (OA) from water, especially near its isoelectric point. A corrected model accounts for protein denaturation during bubble interactions, explaining residual concentrations in wastewater treatment.
Area of Science:
- Environmental Engineering
- Water Treatment Technologies
- Separation Processes
Background:
- Wastewater treatment requires efficient methods to reduce organic loading.
- Protein removal is crucial for mitigating environmental pollution.
- Foam separation offers a potential technique for protein removal from aqueous solutions.
Purpose of the Study:
- To investigate the efficiency of batch foam separation for removing ovalbumin (OA) from water.
- To develop and validate a mathematical model for predicting OA removal during foam separation.
- To understand the factors influencing residual OA concentrations and protein denaturation.
Main Methods:
- Batch foam separation experiments using ovalbumin (OA) as a model protein.
- Optimization of removal efficiency by adjusting pH to the isoelectric point (pI) of OA (pH 4.6).
- Development of a mass balance model and a corrected model incorporating surface denaturation effects.
Main Results:
- Maximum OA removal efficiency was observed near its isoelectric point (pH 4.6).
- A plateau residual OA concentration of approximately 18% of the initial concentration was experimentally observed.
- The corrected model, including protein denaturation at the bubble interface, accurately predicted the OA concentration profile over time.
Conclusions:
- Batch foam separation is a viable method for removing proteins like OA from wastewater.
- Protein denaturation at the bubble surface is a significant factor limiting complete removal.
- The corrected model provides a better understanding of the foam separation process dynamics for protein removal.
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