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RETRACTED: Simultaneous determination of alkaloids, diterpenoids and steroids in Jin-Guo-Lan by high performance

Yu Feng Zhang1, Peiying Shi, Qiyong Shi

  • 1Pharmaceutical Informatics Institute, Zhejiang University, No. 38 Zheda Road, Hangzhou, Zhejiang Provine 310027, PR China.

Journal of Pharmaceutical and Biomedical Analysis
|September 9, 2006
PubMed

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High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process: