Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

GPI Anchoring of Proteins in the ER Membrane01:29

GPI Anchoring of Proteins in the ER Membrane

GPI-anchoring is a post-translational, reversible protein modification that is ubiquitous in eukaryotes. Such proteins are primarily present on the exoplasmic leaflet of the plasma membrane.
GPI-anchor structure
A sequence of 11 enzymatic reactions results in the synthesis of the complete GPI anchor consisting of a hydrophobic and a hydrophilic portion. The hydrophobic portion comprises phosphatidylinositol, while the hydrophilic part comprises polar groups like phosphoethanolamine,...
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Size and Structure of Viral Genomes01:26

Size and Structure of Viral Genomes

Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chronotherapeutic administration of neoadjuvant chemotherapy reduces cancer-related fatigue-like behavior.

Brain, behavior, & immunity - health·2026
Same author

Nile red spectroscopy to evaluate disease-specific lipid alterations in glomerular kidney disease.

Methods and applications in fluorescence·2026
Same author

Generation of Schlafen 8-Specific Antibodies.

Antibodies (Basel, Switzerland)·2026
Same author

Spatial transcriptomics identifies IL-32 as a lipid droplet-associated cytokine linked to tubular injury in human diabetic kidney disease.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]·2026
Same author

G-Protein-Coupled Receptor Kinase 2 Limits CCL21-Induced T Cell Migration via Phospholipase Cγ1.

Receptors (Basel, Switzerland)·2026
Same author

ADAR1 haploinsufficiency and sustained picornaviral RdRp dsRNA synthesis synergize to dysregulate RNA editing and cause multi-system interferonopathy.

mBio·2025

Related Experiment Video

Updated: Jul 18, 2026

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
11:28

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

Published on: October 7, 2011

An essential role for LEDGF/p75 in HIV integration.

Manuel Llano1, Dyana T Saenz, Anne Meehan

  • 1Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.

Science (New York, N.Y.)
|September 9, 2006
PubMed
Summary

Lens epithelium-derived growth factor (LEDGF)/p75 is crucial for human immunodeficiency virus (HIV) integration into the host genome. This essential cofactor links viral integrase to chromatin, a key step for establishing the permanent HIV reservoir.

More Related Videos

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library
07:28

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

Published on: January 10, 2025

Visualization and Quantitative Analysis of Genotoxin-Induced PARP1/PARP2 Activation in Cells Using a Fluorescent Fusion Protein-Based Reporter
07:53

Visualization and Quantitative Analysis of Genotoxin-Induced PARP1/PARP2 Activation in Cells Using a Fluorescent Fusion Protein-Based Reporter

Published on: April 17, 2026

Related Experiment Videos

Last Updated: Jul 18, 2026

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
11:28

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

Published on: October 7, 2011

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library
07:28

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

Published on: January 10, 2025

Visualization and Quantitative Analysis of Genotoxin-Induced PARP1/PARP2 Activation in Cells Using a Fluorescent Fusion Protein-Based Reporter
07:53

Visualization and Quantitative Analysis of Genotoxin-Induced PARP1/PARP2 Activation in Cells Using a Fluorescent Fusion Protein-Based Reporter

Published on: April 17, 2026

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Human immunodeficiency virus (HIV) establishes a permanent reservoir through chromosomal integration.
  • The role of cellular proteins in HIV integration is not fully understood.
  • Understanding these factors is key to developing strategies for HIV eradication.

Purpose of the Study:

  • To identify essential cellular cofactors involved in HIV chromosomal integration.
  • To elucidate the mechanism by which cellular proteins facilitate HIV integration.
  • To explore potential therapeutic targets for disrupting HIV replication.

Main Methods:

  • Intensified RNA interference (RNAi) screens to identify host factors.
  • Dominant-negative protein expression to inhibit specific protein functions.
  • Biochemical assays to study protein-protein and protein-DNA interactions.

Main Results:

  • Lens epithelium-derived growth factor (LEDGF)/p75 was identified as an essential cofactor for HIV integration.
  • LEDGF/p75 functions by forming a molecular tether between viral integrase and host cell chromatin.
  • Very low levels of endogenous LEDGF/p75 are sufficient for integration, potentially explaining why it was previously overlooked.

Conclusions:

  • LEDGF/p75 is a critical cellular protein required for HIV integration.
  • The interaction between LEDGF/p75 and HIV integrase is a potential target for novel antiviral therapies.
  • Targeting this interaction could offer a new strategy to combat the establishment of the HIV reservoir.