Related Experiment Videos
On the use of T7 RNA polymerase transcripts for physical investigation
A A Szewczak1, S A White, D T Gewirth
1Department of Chemistry, Yale University, New Haven, CT 06511.
Nucleic Acids Research
|July 25, 1990
Abstract:
A few years ago we made some observations which raised questions about the accuracy with which T7 RNA polymerase transcribes templates in vitro, and the suitability of its in vitro products for biophysical study (1). The experiments described below demonstrate that there is no reason for concern; the products of T7 RNA polymerase transcription in vitro are as suitable for biophysical characterization as RNAs synthesized in vivo. It is likely that aggregation involving the transcribed portions of the T7 RNA polymerase promoter caused our initial observations.